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By W. N. Aldridge (eds.)

ISBN-10: 1349010855

ISBN-13: 9781349010851

ISBN-10: 1349010871

ISBN-13: 9781349010875

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Meister, R. A. Ronzio, W. B. Rowe, J. D. Gass, & S. L. N. '\.. -· w....... 1:. ,, 20 ) .... , 0 Time(minl FIG. 8. Effect of glutamate, ammonia and hydroxylamine on the inhibition of glutamine synthetase by methionine sulphoximine. (a) Glutamate and NH 3 : curve 1 (0-0): a reaction mixture (0·1 ml) containing enzyme (3·8 (LM), MgC12 (0·02 M), 2-mercaptoethanol (0·01 M), and imidazole-HCl buffer {0·05 M, pH 7·2) was placed at 37°C for 5 min. Then a solution (0·002 ml) containing L-methionine sulphoximine (0·025 M) and NaATP (0·5 M) was added, and the mixture was incubated at 37°C.

LEESON, G. & ScoTT, C. C. (1959). Ann. Y. Acad. , 80, 597 BIEL, Monoamine Oxidase Inhibitors 27 CHRISTMAS, A. , HALL, D. w. , HAYWARD, A. & MAXWELL, D. R. (1970). Br. J. , in the Press CLINESCHMIDT, B. V. & HoRITA, A. (1969). Biochem. , 18, 1011, 1021 CoLLINS, G. G. , YoUDIM, M. B. H. & SANDLER, M. (1968). , 1, 215 CoLLINS, G. G. , WILLIAMS, E. D. & YoumM, M. B. H. (1970). , SHAW, D. , HERZBERG, B. & Muoos, R. (1970). Lancet, 2, 1178 CREASEY, N. H. (1956). Biochem. , 64, 178 DAVISON, A. N. (1957).

Rao TABLE 1. Summary of specificity of glutamine synthetase Compound Active form Glutamic acid a-Methyl glutamic acid ,8-Methyl glutamic acid y-Methyl glutamic acid ,8-Glutamic acid Land D Only L Only threo-D Only threo-L Only o (product) substrate. Of the four y-methylglutamic acids, only threo-y-methyl-Lglutamic acid is a substrate. Fig. 4 gives a diagram showing the extended conformations of L- and o-glutamic acid in the orientations postulated at the active site of the enzyme; only three hydrogen atoms may be substituted by a methyl group without loss of enzymatic susceptibility.

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A Symposium on Mechanisms of Toxicity by W. N. Aldridge (eds.)


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