Download e-book for iPad: Advances in Lipid Methodology. Volume 3 by W. W. Christie

By W. W. Christie

ISBN-10: 0857098004

ISBN-13: 9780857098009

ISBN-10: 0951417169

ISBN-13: 9780951417164

This is often the 3rd quantity of an occasional sequence of assessment volumes facing points of lipid technique. As with the 1st volumes, themes were chosen which were constructing swiftly lately and feature a few significance to lipid research. The authors are all prime overseas experts.

Topics coated contain: research of positional isomers of glycerolipids via non-enzymatic equipment, separation of phospholipid periods via high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy and lipid section behaviour and lipid diffusion, between others

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Extra resources for Advances in Lipid Methodology. Volume 3

Example text

J. , 36, 1046-1057 (1995). J. , J. , 36, 125-136 (1995). , Lorbeer,E. , Org. , 29, 253-259 (1994). Zollner,F. ,J. , 30, 432-437 (1995). Chapter2 NUCLEAR MAGNETIC RESONANCE PROFILING OF PHOSPHOLIPIDS 31P Thomas Glonek1 and Thomas E. Merchant2 1MR Laboratory, Midwestern University, Chicago, Illinois 60615; 2Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA. A. Introduction B. Total Lipid Extraction C. A 31 P NMR Phospholipid Reagent 1. A hydrated chloroform-methanol reagent for 31 P NMR analysis 2.

Malinski,E. , lipids, 21, 434439 (1986). 21. D. , lipids, 22, 362-365 (1987). 22. A. , Lipids, 26, 769-773 (1991). 23. E. , Lipids, 25, 40-47 (1990). 24. , Ohlson,R. , Chem. Phys. lipids, 14, 174-188 (1975). 25. , in Advances in Lipid Methodology - Two, pp. W. Christie, Oily Press, Ayr, Scotland). 26. ,J. Am. Oil Chem. , 70, 361-366 (1993). 27. , Chromatography for the Analysis ofLipids, pp. 113-168 ( 1993). 28. ltabashi,Y. , Lipids, 21, 413-416 (1986). 29. ltabashi,Y. ,J. , 402, 257-264 (1987).

Nineteen phospholipids were detected, and eighteen of these were identified. The signal resolution shown in the figure can be obtained with any properly prepared tissue phospholipid extract. This figure may be compared to earlier attempts to use 31 P NMR for phospholipid analysis. 1. 2. Effect ofphospholipid concentration on phosphorus chemical shifts The chemical shifts of some of the phospholipids vary to a small degree, depending on the concentration of total lipid in the reagent. These shift differences are particularly noticeable when analysis is attempted of extremely dilute ADVANCES IN LIPID METHODOLOGY -THREE 41 AAPC ""' EPLAS ""' JC DPG '\ LPE DHSM DPG AAPE PA LPS )'I LAAPC PG u GPLAS~ \ LPA- 1.

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