By Gary D. Christian
Largely revised and up to date with a extra sleek taste and a brand new, two-color layout, this 6th version offers with ideas and strategies of quantitative research. Examples of analytical innovations are drawn from such parts as lifestyles sciences, medical chemistry, air and water toxins, and commercial analyses. New to this variation: Excel spreadsheets on CD-ROM * New chapters on reliable laboratory perform, in addition to genomics and proteomics * A extra smooth taste.
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Additional info for Analytical Chemistry
And Eizner, Yu. , Sov. Phys. Tech. , 4, 1020 (1960). 9. Pasch, H. , MALDI-TOF Mass Spectrometry of Synthetic Polymers, Springer, Berlin (2003). 1 THEORY AND BASIC PRINCIPLES This chapter describes basic principles of scattering of light by dilute solutions of macromolecules or dispersions of colloidal particles and presents various examples of the application of light scattering for the characterization of unfractionated polymer solutions. Principles and applications of the light scattering hyphenated with a separation technique are presented in Chapters 4 and 5.
The parameters K and a should always be quoted not only with the solvent and temperature, but also with the number of samples and their molar mass range and polydispersity, and the method of the molar mass determination. As a rule of thumb, the values obtained with narrow polymers using light scattering should be preferred. 1 Properties of Mark-Houwink Exponent The literature shows relatively signiﬁcant differences of the values K and a even for polymers measured under identical conditions. The low K values are associated with the high a values and vice versa, which means the differences are to a certain level mutually compensated when the constants are used to calculate the molar mass averages.
At the beginning of polymer science, the empirical distribution functions were often used to estimate the entire distribution from the knowledge of two molar mass averages. With the development of size exclusion chromatography, the importance of the distribution functions as the means of the estimation of molar mass distribution dropped signiﬁcantly, but they still can ﬁnd utilization in theoretical modeling and calculations. In addition, the discrepancy between the experimental and theoretical distribution functions can indicate side reactions.
Analytical Chemistry by Gary D. Christian